GeneTex
  • Country / Location Selection

United States (US)

Rat SLIT2 protein (active)

Rat SLIT2 protein (active). GTX00062-pro
Rat SLIT2 protein (active). GTX00062-pro
Rat SLIT2 protein (active). GTX00062-pro

Cat No. GTX00062-pro

Application Functional Assay
Species Rat
Package
10 μg ($309)
APPLICATION

Application Note

Slit is a family of secreted extracellular matrix proteins which play an important signalling role in the neural development of most bilaterians. Humans, mice and other vertebrates possess three Slit homologs: Slit1, Slit2 and Slit3. The Slit2 protein has recently been discovered to be associated with the development of new blood vessels from pre-existing vessels, or angiogenesis. Slit2 has been implicated in promoting angiogenesis in mice (both in vitro and in vivo), in the human placenta, and in tumorigenesis. Besides, Gremlin 1 (GREM1) has been identified as an interactor of Slit2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Slit2 and recombinant rat GREM1. Briefly, Slit2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to GREM1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-Slit2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of Slit2 and GREM1 was in a dose dependent manner.

Observed MW

20 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asn209~Gly374

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

slit guidance ligand 2

Background

acts as a ligand for glypican-1, a heparan sulfate proteoglycan; may play a role in neurogenesis and midline development [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat SLIT2 protein (active). GTX00062-pro

GTX00062-pro Functional Assay Image

Functional ELISA analysis of GTX00062-pro Rat SLIT2 protein (active) which can bind immobilized GREM1 protein.

Rat SLIT2 protein (active). GTX00062-pro

GTX00062-pro Image

WB analysis of GTX00062-pro Rat SLIT2 protein (active).

Rat SLIT2 protein (active). GTX00062-pro

GTX00062-pro Image

SDS-PAGE analysis of GTX00062-pro Rat SLIT2 protein (active).

REFERENCE
There are currently no references for Rat SLIT2 protein (active) (GTX00062-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Rat SLIT2 protein (active) (GTX00062-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 309