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Rat TGF beta 2 protein (active)

Rat TGF beta 2 protein (active). GTX00377-pro
Rat TGF beta 2 protein (active). GTX00377-pro
Rat TGF beta 2 protein (active). GTX00377-pro

Cat No. GTX00377-pro

Application Functional Assay
Species Rat
Package
10 μg ($319)
APPLICATION

Application Note

Transforming growth factor-beta 2 (TGF-β2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development (alternative names: Glioblastoma-derived T-cell suppressor factor, G-TSF, BSC-1 cell growth inhibitor, Polyergin, Cetermin). It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. Besides, Vitronectin (VTN) has been identified as an interactor of TGF-β2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TGF-β2 and recombinant rat VTN. Briefly, TGF-β2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to VTN-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TGF-β2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TGF-β2 and VTN was in a dose dependent manner.

Observed MW

12 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala331~Ser442 (NP_112393.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

transforming growth factor, beta 2,TGF-B2

Background

binds the transforming growth factor-beta receptor; plays a role in regulation of cell growth and proliferation; may be involved in mesenchymal-epithelial cell interactions during development [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat TGF beta 2 protein (active). GTX00377-pro

GTX00377-pro Functional Assay Image

Functional ELISA analysis of GTX00377-pro Rat TGF beta 2 protein (active) which can bind immobilized VTN protein.

Rat TGF beta 2 protein (active). GTX00377-pro

GTX00377-pro Image

WB analysis of GTX00377-pro Rat TGF beta 2 protein (active).

Rat TGF beta 2 protein (active). GTX00377-pro

GTX00377-pro Image

SDS-PAGE analysis of GTX00377-pro Rat TGF beta 2 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 319