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Rat TIMP3 protein (active)

Rat TIMP3 protein (active). GTX00064-pro
Rat TIMP3 protein (active). GTX00064-pro
Rat TIMP3 protein (active). GTX00064-pro

Cat No. GTX00064-pro

Application Functional Assay
Species Rat
Package
10 μg ($289)
APPLICATION

Application Note

Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) is an protein belongs to the tissue inhibitor of metalloproteinases family. They are inhibitors of the matrix metalloproteinases. TIMP-3 is the only member of the TIMP family which is found exclusively in the extracellular matrix (ECM). It is regulated in a cell cycle-dependent fashion in certain cell types and may serve as a marker for terminal differentiation. Besides, Matrix Metalloproteinase 2 (MMP2) has been identified as an interactor of TIMP3, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TIMP3 and recombinant rat MMP2. Briefly, TIMP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to MMP2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TIMP3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TIMP3 and MMP2 was in a dose dependent manner.

Observed MW

22 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser27~Thr209

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TIMP metallopeptidase inhibitor 3,Timp-3

Background

may inhibit metalloendopeptidase activity; may play a role in induction of apoptosis [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat TIMP3 protein (active). GTX00064-pro

GTX00064-pro Functional Assay Image

Functional ELISA analysis of GTX00064-pro Rat TIMP3 protein (active) which can bind immobilized MMP2 protein.

Rat TIMP3 protein (active). GTX00064-pro

GTX00064-pro Image

WB analysis of GTX00064-pro Rat TIMP3 protein (active).

Rat TIMP3 protein (active). GTX00064-pro

GTX00064-pro Image

SDS-PAGE analysis of GTX00064-pro Rat TIMP3 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289