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Rat TIMP3 protein (active)

Rat TIMP3 protein (active). GTX00064-pro
Rat TIMP3 protein (active). GTX00064-pro
Rat TIMP3 protein (active). GTX00064-pro

Cat No. GTX00064-pro

Application Functional Assay
Species Rat
Package
10 μg ($289)
APPLICATION

Application Note

Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) is an protein belongs to the tissue inhibitor of metalloproteinases family. They are inhibitors of the matrix metalloproteinases. TIMP-3 is the only member of the TIMP family which is found exclusively in the extracellular matrix (ECM). It is regulated in a cell cycle-dependent fashion in certain cell types and may serve as a marker for terminal differentiation. Besides, Matrix Metalloproteinase 2 (MMP2) has been identified as an interactor of TIMP3, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TIMP3 and recombinant rat MMP2. Briefly, TIMP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to MMP2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TIMP3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TIMP3 and MMP2 was in a dose dependent manner.

Observed MW

22 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser27~Thr209

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TIMP metallopeptidase inhibitor 3 , Timp-3

Background

may inhibit metalloendopeptidase activity; may play a role in induction of apoptosis [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat TIMP3 protein (active). GTX00064-pro

GTX00064-pro Functional Assay Image

Functional ELISA analysis of GTX00064-pro Rat TIMP3 protein (active) which can bind immobilized MMP2 protein.

Rat TIMP3 protein (active). GTX00064-pro

GTX00064-pro Image

WB analysis of GTX00064-pro Rat TIMP3 protein (active).

Rat TIMP3 protein (active). GTX00064-pro

GTX00064-pro Image

SDS-PAGE analysis of GTX00064-pro Rat TIMP3 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289