GeneTex
  • Country / Location Selection

United States (US)

Rat TNF alpha protein, His tag (active)

Rat TNF alpha protein, His tag (active). GTX67168-pro

Cat No. GTX67168-pro

Application

Functional Assay

Species

Rat
Package
20 μg ($219)

APPLICATION

Application Note

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is less or equal to 0.2 ng/ml.

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4) with 10% glycerol, 1 mM DTT.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Region/Sequence

N-terminal His-Tag; Length: 181 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMLRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L

Expression System

E.coli

Purity

> 90% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor necrosis factor , RATTNF , TNF-alpha , Tnfa

Background

acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat TNF alpha protein, His tag (active). GTX67168-pro

GTX67168-pro Image

3 μg of GTX67168-pro Rat TNF alpha protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

There are currently no references for Rat TNF alpha protein, His tag (active) (GTX67168-pro). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Rat TNF alpha protein, His tag (active) (GTX67168-pro). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 219