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Rat TNF alpha protein, His tag (active)

Rat TNF alpha protein, His tag (active). GTX67168-pro

Cat. No. GTX67168-pro

Application

 Functional Assay

Species

 Rat
Package
20 μg ($219)
See all TNF alpha protein products

APPLICATION

Application Note

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is less or equal to 0.2 ng/ml.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol, 1mM DTT

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

N-terminal His-Tag; Length: 181 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMLRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L

Expression System

E. coli

Purity

> 90% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor necrosis factor , RATTNF , TNF-alpha , Tnfa

Background

acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat TNF alpha protein, His tag (active). GTX67168-pro

GTX67168-pro Image

3 μg of GTX67168-pro Rat TNF alpha protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 219