GeneTex

  • Country / Location Selection

United States (US)

Rat TNF alpha protein, His tag (active)

Rat TNF alpha protein, His tag (active). GTX00342-pro
Rat TNF alpha protein, His tag (active). GTX00342-pro
Rat TNF alpha protein, His tag (active). GTX00342-pro

Cat. No. GTX00342-pro

Application

 Functional Assay

Species

 Rat
Package
10 μg ($249)
See all TNF alpha protein products

APPLICATION

Application Note

TNF-a (Tumor necrosis factor), being an endogenous pyrogen, is able to induce fever, apoptotic cell death, inflammation and to inhibit tumorigenesis. As reported, TNFa could inhibit the proliferation and induce apoptosis of A549 cells, and the concentration of IL-1β and IL-8 in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNFa (1 ng/ml, 10 ng/ml, 100 ng/ml, 1000 ng/ml) for 8h, IL-1β and IL-8 were detected in the cell supernatant by ELISA. After incubation with TNF-a for 8h, IL-1β significantly increased in the cell supernatant.

Observed MW

21 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu80~Leu235 (NP_036807.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor necrosis factor , RATTNF , TNF-alpha , Tnfa

Background

acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat TNF alpha protein, His tag (active). GTX00342-pro

GTX00342-pro Functional Assay Image

ELISA detection of secreted IL-6 protein activation from A549 cells induced by GTX00342-pro Rat TNF alpha protein (active) for 8hrs.
Recombinant protein concentrations: 1, 10, 100, and 1000 ng/ml.

Rat TNF alpha protein, His tag (active). GTX00342-pro

GTX00342-pro Image

SDS-PAGE analysis of GTX00342-pro Rat TNF alpha protein (active).

Rat TNF alpha protein, His tag (active). GTX00342-pro

GTX00342-pro Image

WB analysis of GTX00342-pro Rat TNF alpha protein (active).

REFERENCE

There are currently no references for Rat TNF alpha protein, His tag (active) (GTX00342-pro). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Rat TNF alpha protein, His tag (active) (GTX00342-pro). Be the first to share your experience with this product.
Submit a Review
SDS
Proclin.pdf
Package List Price ($)
$ 249