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Rat VEGFA protein (active)

Rat VEGFA protein (active). GTX00343-pro
Rat VEGFA protein (active). GTX00343-pro
Rat VEGFA protein (active). GTX00343-pro
Rat VEGFA protein (active). GTX00343-pro

Cat No. GTX00343-pro

Application Functional Assay
Species Rat
Package
10 μg ($259)
APPLICATION

Application Note

Vascular endothelial growth factor A (VEGF-A), a glycosylated mitogen, is known to be a vascular permeability factor and an endothelial cell growth factor secreted by the smooth muscle and endothelial cells. It has been reported that VEGF-A induces vascular permeability and growth, promotes monocyte/macrophage migration, and inhibits cell apoptosis and so on. To test the effect of VEGF-A on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of VEGFA. After incubated for 72h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37ºC . Cell proliferation of ECV304 cells after incubation with VEGFA for 72h observed by inverted microscope. VEGFA significantly promoted cell proliferation of ECV304 cells. The ED₅₀ for this effect is typically 5. 58-9. 98 ng/ml.

Observed MW

22 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.4) with 0.01% SKL, 1 mM DTT, 5% Trehalose, Proclin300. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala27~Arg190 (NP_001103804.1)

Expression System

293F cell

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

vascular endothelial growth factor A , VEGF-A , VEGF111 , VEGF164 , VPF , Vegf

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES
Rat VEGFA protein (active). GTX00343-pro

GTX00343-pro Functional Assay Image

The dose effect curve of GTX00343-pro Rat VEGFA protein (active) on ECV-304 cells. After incubation of Rat VEGFA protein (active) for 72 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Rat VEGFA protein (active). GTX00343-pro

GTX00343-pro Functional Assay Image

Cell proliferation effect of GTX00343-pro Rat VEGFA protein (active).
(A) Unstimulated ECV-304 cells cultured in serum-free DMEM for 72hrs.
(B) ECV-304 cells cultured in serum-free DMEM, stimulated with 10 ng/ml VEGFA for 72hrs.

Rat VEGFA protein (active). GTX00343-pro

GTX00343-pro Image

WB analysis of GTX00343-pro Rat VEGFA protein (active).

Rat VEGFA protein (active). GTX00343-pro

GTX00343-pro Image

SDS-PAGE analysis of GTX00343-pro Rat VEGFA protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 259