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Rb antibody [13A10]

Anti-Rb antibody [13A10] used in IHC (Paraffin sections) (IHC-P). GTX01934

Cat. No. GTX01934

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 13A10

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human
Package
500 μl ($649)
See all Rb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:25–1:50
IHC-P 1:25–1:50
IHC-Fr Assay dependent

Note :

IHC-P
High temperature antigen unmasking technique. Standard ABC technique.

IHC-Fr
Optimum fixative, Zamboni's 10 minutes at 25ºC (see Stefanini et al., 1967).
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Antigen Species

Human

Immunogen

Recombinant prokaryotic protein corresponding to a portion of the N-terminal region of the retinoblastoma gene product.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rb antibody [13A10] used in IHC (Paraffin sections) (IHC-P). GTX01934

GTX01934 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX01934 Rb antibody [13A10]. Note intense nuclear staining of epithelial cells.

REFERENCE

Clone Reference

T Hayashi et al. Pathol Int 2020; 70 (5):295-299 Transformation from EGFR/PTEN co-mutated lung adenocarcinoma to small cell carcinoma in lymph node metastasis
Application : IHC-P
Reactivity : Human
B Hinds et al. J Cutan Pathol 2017; 44 (8):665-671 Loss of retinoblastoma in pleomorphic fibroma: An immunohistochemical and genomic analysis
Application : IHC-P
Reactivity : Human
Weiqiang Zhao et al. J Oncol 2012; (2012):957437 Altered p16(INK4) and RB1 Expressions Are Associated with Poor Prognosis in Patients with Nonsmall Cell Lung Cancer
Application : IHC-P
Reactivity : Human
K Bhatia et al. Int J Cancer 2010; 127 (6):1493-6 Immunological detection of viral large T antigen identifies a subset of Merkel cell carcinoma tumors with higher viral abundance and better clinical outcome
PP Sedghizadeh et al. Head Neck 2006; 28 (5):432-40 Expression of the serine protease DESC1 correlates directly with normal keratinocyte differentiation and inversely with head and neck squamous cell carcinoma progression
Application : IHC-P
Reactivity : Human
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Package List Price ($)
$ 649