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Rb antibody [1F8]

Anti-Rb antibody [1F8] used in IHC (Paraffin sections) (IHC-P). GTX34996

Cat. No. GTX34996

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μg ($529)
See all Rb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF Assay dependent
IHC-P 1-2μg/ml for 30 minutes at RT
IP 1-2μg/500ug protein lysate

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Product Note

Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Rb1 protein fragment of 283 amino acid residues.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

OSRC , PPP1R130 , RB , RB transcriptional corepressor 1 , RB1 , p105Rb , pRb , pp110 , Rb

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rb antibody [1F8] used in IHC (Paraffin sections) (IHC-P). GTX34996

GTX34996 IHC-P Image

IHC-P analysis of human colon tissue using GTX34996 Rb antibody [1F8].

REFERENCE

Clone Reference

L Montanaro et al. Cell Prolif . 2007; 40 (4) Different effects of ribosome biogenesis inhibition on cell proliferation in retinoblastoma protein- and p53-deficient and proficient human osteosarcoma cell lines
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529