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Rb antibody, C-term

Anti-Rb antibody, C-term used in Western Blot (WB). GTX81164
Anti-Rb antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81164
Anti-Rb antibody, C-term used in Western Blot (WB). GTX81164
Anti-Rb antibody, C-term used in Flow cytometry (FACS). GTX81164

Cat. No. GTX81164

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all Rb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 858-886 amino acids from the C-terminal region of human RB1.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rb antibody, C-term used in Western Blot (WB). GTX81164

GTX81164 WB Image

WB analysis of 293 cell lysate (35ug/lane) using GTX81164 Rb antibody, C-term.

Anti-Rb antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81164

GTX81164 IHC-P Image

IHC-P analysis of human testis tissue using GTX81164 Rb antibody, C-term.

Anti-Rb antibody, C-term used in Western Blot (WB). GTX81164

GTX81164 WB Image

WB analysis of various samples using GTX81164 Rb antibody, C-term.
Lane 1: HeLa whole cell lysate
Lane 2: Jurkat whole cell lysate
Loading : 20 μg per lane
Dilution : 1:8000

Anti-Rb antibody, C-term used in Flow cytometry (FACS). GTX81164

GTX81164 FACS Image

FACS analysis of 293 cells using GTX81164 Rb antibody, C-term.
Top histogram : negative control
Bottom histogram : 293 cells

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399