GeneTex

  • Country / Location Selection

United States (US)

Rb antibody [HL2465]

Recombinant antibody
Anti-Rb antibody [HL2465] used in Western Blot (WB). GTX638814
Anti-Rb antibody [HL2465] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638814
Anti-Rb antibody [HL2465] used in Western Blot (WB). GTX638814

Cat. No. GTX638814

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2465

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Rb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent

Note :

WB
We would recommend using "Hot 1% SDS lysis buffer" to collect the sample lysates.
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Rb.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rb antibody [HL2465] used in Western Blot (WB). GTX638814

GTX638814 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rb antibody [HL2465] (GTX638814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rb antibody [HL2465] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638814

GTX638814 ICC/IF Image

Rb antibody [HL2465] detects Rb protein at nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rb stained by Rb antibody [HL2465] (GTX638814) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Rb antibody [HL2465] used in Western Blot (WB). GTX638814

GTX638814 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rb antibody [HL2465] (GTX638814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

There are currently no references for Rb antibody [HL2465] (GTX638814). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Rb antibody [HL2465] (GTX638814). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations