GeneTex
United States (US)

Rb antibody

IP/MS
GTX100545_41297_IHC_19011506_914.jpg
GTX100545_41297_IP_19011506_637.jpg
GTX100545_41297_20151216_IFA_19011506_937.jpg
GTX100545_41297_WB_19011506_595.jpg
GTX100545_43250_20180706_WB_19011506_137.jpg

Cat No. GTX100545

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Positive Control

Jurkat , A431 , *CL1-0 , *CL1-5

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377374)

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Rb. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

OSRC antibody, RB antibody, p105-Rb antibody, pRb antibody, pp110 antibody, RB1 antibody, retinoblastoma suspectibility protein antibody, retinoblastoma-associated protein antibody, retinoblastoma 1 antibody

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100545_41297_IHC_19011506_914.jpg

GTX100545 IHC-P Image

Rb antibody detects Rb protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Rb antibody (GTX100545) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100545_41297_IP_19011506_637.jpg

GTX100545 IP Image

Rb antibody immunoprecipitates retinoblastoma 1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of retinoblastoma 1 protein by 4 μg Rb antibody (GTX100545)
7.5 % SDS-PAGE
The immunoprecipitated retinoblastoma 1 protein was detected by Rb antibody (GTX100545) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX100545_41297_20151216_IFA_19011506_937.jpg

GTX100545 ICC/IF Image

Rb antibody detects Rb protein at nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rb protein stained by Rb antibody (GTX100545) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX100545_41297_WB_19011506_595.jpg

GTX100545 WB Image

Rb antibody detects Rb protein by western blot analysis.
A. 30 µg A431 whole cell lysate/extract
5% SDS-PAGE
Rb antibody (GTX100545) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100545_43250_20180706_WB_19011506_137.jpg

GTX100545 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rb antibody (GTX100545) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Rb antibody Cat No. GTX100545

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 15-Jul-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : CL1-0 cells, CL1-5 cells
Amount used : 30μg
Blocking : 3% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane1:CL1-5 cells, Lane2:CL1-0 cells
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf