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Rb cleaved antibody [172C1094]

Anti-Rb cleaved antibody [172C1094] used in Western Blot (WB). GTX13905

Cat. No. GTX13905

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 172C1094

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all Rb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
IHC-P Assay dependent
IHC-Fr 1:10 - 1:500
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Product Note

The antibody recognizes cleaved Rb (DRb-p70). The antibody does not recognize full-length Rb.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a region of the caspase-3-induced cleavage site of human Rb was used as immunogen.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_369560

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rb cleaved antibody [172C1094] used in Western Blot (WB). GTX13905

GTX13905 WB Image

WB analysis of HL-60 cells treated with camptothecin for 24 hours using GTX13905 Rb cleaved antibody [172C1094].

REFERENCE

Application Reference

Liu HP et al. Cell Prolif 2005; 38 (1) : 13-24 A novel form of pRb expressed during normal myelopoiesis and in tumour-associated macrophages.
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399