WB (peptide competition) analysis of Jurkat cells in high growth phase using GTX24788 Rb (phospho Ser249) antibody prior incubated with a generic phosphoserine-containing peptide (Lane 2), a generic phosphothreonine- containing peptide (Lane 3), the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

WB analysis of whole cell extracts (30 μg lysate) of Jurkat (Lane 1), Serum Starved Jurkat (Lane 2), Serum Starved for overnight following by serum release (Lane 3), and Jurkat treated for 15 min with 25 ng/ml of Calyculin A (lane 4) using GTX24788 Rb (phospho Ser249) antibody.
Dilution : 1:500-1:2000