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Rb (phospho Ser780) antibody

Orthogonal
Anti-Rb (phospho Ser780) antibody used in Western Blot (WB). GTX133893

Cat. No. GTX133893

Reference 1
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Rb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser780 of human Rb. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887134

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rb (phospho Ser780) antibody used in Western Blot (WB). GTX133893

GTX133893 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Rb (phospho Ser780) antibody (GTX133893) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chen HL et al. Anticancer Res 2020; 40 (9) : 4947-4960 Effects of Sclareol Against Small Cell Lung Carcinoma and the Related Mechanism: In Vitro and In Vivo Studies.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

Rb (phospho Ser780) antibody Cat. No. GTX133893

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-Rb (phospho Ser780) antibody used in Western Blot (WB). GTX133893
Date : Anonymous submitted on 05-Feb-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : EO771 / MDA-MB 231 cells cells with/without treatment
Amount used : 25μg
Blocking : 5% skim milk in TBST, RT°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Rb (phospho Ser780) antibody used in Western Blot (WB). GTX133893
Date : Anonymous submitted on 23-Feb-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 40 μg
Blocking : 0.1% skim milk, 24°C, 1Hr
Primary Antibody : 1:2000, 24°C, 2Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169