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Rb (phospho Ser780) antibody

Anti-Rb (phospho Ser780) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32254
Anti-Rb (phospho Ser780) antibody used in IHC (Paraffin sections) (IHC-P). GTX32254
Anti-Rb (phospho Ser780) antibody used in Western Blot (WB). GTX32254

Cat No. GTX32254

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

106 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of Rb. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rb Transcriptional Corepressor 1,Osrc,Ppp1R130,Rb,P105-Rb,Prb,Pp110,Rb1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Rb (phospho Ser780) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32254

GTX32254 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX32254 Rb (phospho Ser780) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-Rb (phospho Ser780) antibody used in IHC (Paraffin sections) (IHC-P). GTX32254

GTX32254 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32254 Rb (phospho Ser780) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-Rb (phospho Ser780) antibody used in Western Blot (WB). GTX32254

GTX32254 WB Image

WB analysis of FBS-treated Jurkat (A), HeLa (B) whole cell lysates using GTX32254 Rb (phospho Ser780) antibody.