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Rb (phospho Ser780) antibody

Anti-Rb (phospho Ser780) antibody used in Western Blot (WB). GTX50193
Anti-Rb (phospho Ser780) antibody used in IHC (Paraffin sections) (IHC-P). GTX50193
Anti-Rb (phospho Ser780) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50193

Cat No. GTX50193

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 780 (T-L-S(p)-P-I) derived from human Rb.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rb (phospho Ser780) antibody used in Western Blot (WB). GTX50193

GTX50193 WB Image

WB analysis of extracts from 3T3 cells untreated or treated with UV using GTX50193 Rb (phospho Ser780) antibody.

Anti-Rb (phospho Ser780) antibody used in IHC (Paraffin sections) (IHC-P). GTX50193

GTX50193 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50193 Rb (phospho Ser780) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Rb (phospho Ser780) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50193

GTX50193 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50193 Rb (phospho Ser780) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf