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Rb (phospho Ser780) antibody

Anti-Rb (phospho Ser780) antibody used in IHC (Paraffin sections) (IHC-P). GTX78952
Anti-Rb (phospho Ser780) antibody used in Western Blot (WB). GTX78952

Cat. No. GTX78952

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 780 (T-L-Sp-P-I).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11178872

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

OSRC,PPP1R130,RB,RB transcriptional corepressor 1,RB1,p105Rb,pRb,pp110,Rb

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rb (phospho Ser780) antibody used in IHC (Paraffin sections) (IHC-P). GTX78952

GTX78952 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX78952 Rb (phospho Ser780) antibody.

Anti-Rb (phospho Ser780) antibody used in Western Blot (WB). GTX78952

GTX78952 WB Image

WB analysis of K562 cells untreated or treated with 10% serum (after 48 hours of starvation) lysates using GTX78952 Rb (phospho Ser780) antibody (Lane 3 and 4) and Rb antibody (Lane 1 and 2).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399