Rb (phospho Ser780) antibody

Cat. No. GTX78952

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μg ($399)

See all Rb products

Datasheet

View product citations for antibody GTX78952 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
IHC-P	1:50 - 1:100

Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 780 (T-L-Sp-P-I).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11178872

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

OSRC,PPP1R130,RB,RB transcriptional corepressor 1,RB1,p105Rb,pRb,pp110,Rb

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Gene ID: 5925 RB1
UniProt: P06400 RB1

Research Area

DATA IMAGES

GTX78952 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX78952 Rb (phospho Ser780) antibody.

GTX78952 WB Image

WB analysis of K562 cells untreated or treated with 10% serum (after 48 hours of starvation) lysates using GTX78952 Rb (phospho Ser780) antibody (Lane 3 and 4) and Rb antibody (Lane 1 and 2).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 399

Qty