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Rb (phospho Ser795) antibody

Orthogonal
Anti-Rb (phospho Ser795) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX38604
Anti-Rb (phospho Ser795) antibody used in Western Blot (WB). GTX38604
Anti-Rb (phospho Ser795) antibody used in IHC (Paraffin sections) (IHC-P). GTX38604

Cat. No. GTX38604

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
50 μl ($399)
See all Rb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Product Note

This antibody detects endogenous levels of Rb only when phosphorylated at serine 795.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 795 (P -S-S(p)-P-L).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rb (phospho Ser795) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX38604

GTX38604 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX38604 Rb (phospho Ser795) antibody.

Anti-Rb (phospho Ser795) antibody used in Western Blot (WB). GTX38604

GTX38604 WB Image

Orthogonal

WB analysis of extracts from K562 cells untreated (lane 1) or treated with serum (lane 2) using GTX38604 Rb (phospho Ser795) antibody.

Anti-Rb (phospho Ser795) antibody used in IHC (Paraffin sections) (IHC-P). GTX38604

GTX38604 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX38604 Rb (phospho Ser795) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399