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United States (US)

Rb (phospho Ser795) antibody

GTX38604_20190129_WB_19012901_418.jpg
GTX38604_20190129_IHC-P_19012901_980.jpg
GTX38604_20190129_ICCIF_19012901_115.jpg

Cat No. GTX38604

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Specificity/Sensitivity

This antibody detects endogenous levels of Rb only when phosphorylated at serine 795.
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg2+ and Ca2+) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 795 (P -S-S(p)-P-L).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rb Transcriptional Corepressor 1,Osrc,Ppp1R130,Rb,P105-Rb,Prb,Pp110,Rb1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX38604_20190129_WB_19012901_418.jpg

GTX38604 WB Image

WB analysis of K562 cell lysate using GTX38604 Rb (phospho Ser795) antibody.

GTX38604_20190129_IHC-P_19012901_980.jpg

GTX38604 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX38604 Rb (phospho Ser795) antibody.

GTX38604_20190129_ICCIF_19012901_115.jpg

GTX38604 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX38604 Rb (phospho Ser795) antibody.