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Rb (phospho Ser795) antibody

Anti-Rb (phospho Ser795) antibody used in IHC (Paraffin sections) (IHC-P). GTX50191
Anti-Rb (phospho Ser795) antibody used in Western Blot (WB). GTX50191
Anti-Rb (phospho Ser795) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50191

Cat. No. GTX50191

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 795 (P-S-S(p)-P-L) derived from human Rb.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rb (phospho Ser795) antibody used in IHC (Paraffin sections) (IHC-P). GTX50191

GTX50191 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50191 Rb (phospho Ser795) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Rb (phospho Ser795) antibody used in Western Blot (WB). GTX50191

GTX50191 WB Image

WB analysis of extracts from K562 cells untreated (lane 1) or treated with serum (lane 2) using GTX50191 Rb (phospho Ser795) antibody.

Anti-Rb (phospho Ser795) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50191

GTX50191 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50191 Rb (phospho Ser795) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399