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Rb (phospho Ser795) antibody

Orthogonal
Anti-Rb (phospho Ser795) antibody used in IHC (Paraffin sections) (IHC-P). GTX78954
Anti-Rb (phospho Ser795) antibody used in Western Blot (WB). GTX78954

Cat. No. GTX78954

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Rb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 795 (P-S-Sp-P-L).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11163059

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

OSRC,PPP1R130,RB,RB transcriptional corepressor 1,RB1,p105Rb,pRb,pp110,Rb

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rb (phospho Ser795) antibody used in IHC (Paraffin sections) (IHC-P). GTX78954

GTX78954 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX78954 Rb (phospho Ser795) antibody.

Anti-Rb (phospho Ser795) antibody used in Western Blot (WB). GTX78954

GTX78954 WB Image

Orthogonal

WB analysis of K562 cells untreated or treated with 10% serum (after 48 hours of starvation) lysates using GTX78954 Rb (phospho Ser795) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399