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Rb (phospho Thr821) antibody [24A7]

Anti-Rb (phospho Thr821) antibody [24A7] used in Western Blot (WB). GTX00682

Cat No. GTX00682

Host

Mouse

Clonality

Monoclonal

Clone Name

24A7

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB ~1 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide containing phospho-Thr821 of human Rb

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rb (phospho Thr821) antibody [24A7] used in Western Blot (WB). GTX00682

GTX00682 WB Image

WB analysis of various samples using GTX00682 Rb (phospho Thr821) antibody [24A7]. The cell extract were immuno-precipitated with anti-Myc antibody and then analyzed by Western Blot with GTX00682 Rb (phospho Thr821) antibody [24A7].
Lane 1 : H1299 overexpressing Myc-tagged wild-type Rb
Lane 2 : H1299 overexpressing Myc-tagged Rb (S795A)
Lane 3 : H1299 overexpressing Myc-tagged Rb (T821A).

REFERENCE

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REVIEW

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