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Rb (phospho Thr826) antibody

Orthogonal
Anti-Rb (phospho Thr826) antibody used in Western Blot (WB). GTX24779
Anti-Rb (phospho Thr826) antibody used in IHC (Paraffin sections) (IHC-P). GTX24779
Anti-Rb (phospho Thr826) antibody used in IHC (Paraffin sections) (IHC-P). GTX24779

Cat. No. GTX24779

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all Rb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:10-1:100
FACS 1:20
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1mg/ml BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Rb that contains threonine 826 (based on Swiss Protein database, accession number P06400). The sequence is conserved in mouse, rat and chicken.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rb (phospho Thr826) antibody used in Western Blot (WB). GTX24779

GTX24779 WB Image

Orthogonal

WB analysis of Jurkat cells in high growth phase using GTX24779 Rb (phospho Thr826) antibody prior incubated with a generic phosphothreonine containing peptide(Lane 2), with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), or the phosphopeptide immunogen (Lane 4). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
Dilution : 0.5mg/ml

Anti-Rb (phospho Thr826) antibody used in IHC (Paraffin sections) (IHC-P). GTX24779

GTX24779 IHC-P Image

IHC-P analysis of human colon tissue using GTX24779 Rb (phospho Thr826) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-Rb (phospho Thr826) antibody used in IHC (Paraffin sections) (IHC-P). GTX24779

GTX24779 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX24779 Rb (phospho Thr826) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399