GeneTex

  • Country / Location Selection

United States (US)

Rb2 / p130 antibody

Orthogonal
Anti-Rb2 / p130 antibody used in IHC (Paraffin sections) (IHC-P). GTX110329
Anti-Rb2 / p130 antibody used in IHC (Paraffin sections) (IHC-P). GTX110329
Anti-Rb2 / p130 antibody used in Western Blot (WB). GTX110329
Anti-Rb2 / p130 antibody used in IHC (Paraffin sections) (IHC-P). GTX110329
Anti-Rb2 / p130 antibody used in Western Blot (WB). GTX110329

Cat. No. GTX110329

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Rb2 / p130 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

Jurkat , Jurkat nuclear

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rb2 / p130. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885278

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB transcriptional corepressor like 2 , P130 , Rb2

Cellular Localization

Nucleus

Background

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

Database

Research Area

DATA IMAGES

Anti-Rb2 / p130 antibody used in IHC (Paraffin sections) (IHC-P). GTX110329

GTX110329 IHC-P Image

p130 antibody detects p130 protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
p130 antibody (GTX110329) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rb2 / p130 antibody used in IHC (Paraffin sections) (IHC-P). GTX110329

GTX110329 IHC-P Image

p130 antibody detects p130 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
p130 antibody (GTX110329) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rb2 / p130 antibody used in Western Blot (WB). GTX110329

GTX110329 WB Image

Orthogonal

p130 antibody detects p130 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Jurkat nuclear lysate/extract
5 % SDS-PAGE
p130 antibody (GTX110329) dilution: 1:500

Anti-Rb2 / p130 antibody used in IHC (Paraffin sections) (IHC-P). GTX110329

GTX110329 IHC-P Image

p130 antibody detects p130 protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
p130 antibody (GTX110329) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rb2 / p130 antibody used in Western Blot (WB). GTX110329

GTX110329 WB Image

p130 antibody detects p130 protein by western blot analysis.
A. 30 μg Jurket whole cell lysate/extract
5 % SDS-PAGE
p130 antibody (GTX110329) dilution: 1:500

REFERENCE

There are currently no references for Rb2 / p130 antibody (GTX110329). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Rb2 / p130 antibody (GTX110329). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169