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Rb2 / p130 antibody

Anti-Rb2 / p130 antibody used in Western Blot (WB). GTX26545
Anti-Rb2 / p130 antibody used in IHC (Paraffin sections) (IHC-P). GTX26545
Anti-Rb2 / p130 antibody used in Western Blot (WB). GTX26545

Cat. No. GTX26545

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ELISA

Reactivity

 Mouse
Package
50 μl ($399)
See all Rb2 / p130 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:200-1:1000
IP 1:100
ELISA 1:5000-1:20000
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rb2 (p130) peptide corresponding to a region near the C-terminus of the human protein conjugated to KLH.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB transcriptional corepressor like 2 , P130 , Rb2

Cellular Localization

Nucleus

Background

The product of the Retinoblastoma related human gene RB2 (p130) is highly homologous to the product of the Rb tumor suppressor gene, Rb (p105), especially in the functional pocket region of the protein. Certain carcinoma derived cell lines show low levels of Rbs expression, possibly due to gene rearrangement. In other experiments, a drastic reduction in proliferation is observed upon introduction of active Rb2.

Database

Research Area

DATA IMAGES

Anti-Rb2 / p130 antibody used in Western Blot (WB). GTX26545

GTX26545 WB Image

Western Blot of Rabbit Anti-Rb2 p130 Antibody. Lane 1: HEK 293 pcDNA3. Lane 2: HEK 293 pcDNA3-Rb2wt. Lane 3: HEK 293 pcDNA3-Rb2-PM19. Load: 30 μg per lane. Primary antibody: Anti-Rb2 antibody at 1:250 for overnight at 4ºC. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4ºC. Predicted/Observed size: 130 kDa for p130/Rb2.

Anti-Rb2 / p130 antibody used in IHC (Paraffin sections) (IHC-P). GTX26545

GTX26545 IHC-P Image

Immunohistochemical staining of mouse tissue using anti-pRb2/p130 antiserum.  The staining shows the location of pRb2/p130 in developing mouse tissue.  Other detection systems should yield similar results. Sections were cut at 5-7 μm, mounted on glass and dried overnight at 37ºC.  All sections were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS).  PBS was used for all subsequent washes and for antiserum dilution.  Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum.  Slides were incubated at 20º C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min.  Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20º C.  Diaminobenzidine was used as the final chromogen.  Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.

Anti-Rb2 / p130 antibody used in Western Blot (WB). GTX26545

GTX26545 WB Image

WB analysis of non-transfected and transfected 293T whole cell lysate using GTX26545 Rb2 / p130 antibody.
Loading : 30 μg
Dilution : 1:250

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399