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RbAp46 antibody

Anti-RbAp46 antibody used in Immunoprecipitation (IP). GTX33460
Anti-RbAp46 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33460
Anti-RbAp46 antibody used in Western Blot (WB). GTX33460

Cat No. GTX33460

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human RBBP7 (NP_002884.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RB binding protein 7, chromatin remodeling factor , RbAp46

Cellular Localization

Nucleus

Background

This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-RbAp46 antibody used in Immunoprecipitation (IP). GTX33460

GTX33460 IP Image

IP analysis of MCF7 cell lysate using GTX33460 RbAp46 antibody.
IP reaction : 1μg antibody / 200μg cell lysate
Dilution : 1:1000

Anti-RbAp46 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33460

GTX33460 ICC/IF Image

ICC/IF analysis of MCF-7 cell using GTX33460 RbAp46 antibody.
Blue : DAPI

Anti-RbAp46 antibody used in Western Blot (WB). GTX33460

GTX33460 WB Image

WB analysis of various samples using GTX33460 RbAp46 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 329