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United States (US)

RbAp48 antibody [11G10]

Orthogonal
GTX70232_40004_20151112_WB_Fraction_18122101_798.jpg
GTX70232_40004_ChIP_18122101_183.jpg
RbAp48-antibody-11G10-GTX70232-IHC-1_18122101_259.jpg
RbAp48-antibody-11G10-GTX70232-ICC_IF-1_18122101_485.jpg
GTX70232_40004_20170713_WB_Fraction_18122101_975.jpg

Cat No. GTX70232

Host Mouse
Clonality Monoclonal
Clone Name 11G10
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IP, ChIP assay, Functional Assay, Blocking, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
ChIP assay Assay dependent
Functional Assay Assay dependent
Blocking Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

MOLT4 , HeLa , LB22 , SH-SY5Y , SH-SY5Y nuclear extract
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RBBP4, RBAP48, Q09028, 602923, 5928

Cellular Localization

Nuclear

Background

RbAp48-11G10 recognizes full length RbAp48, a 48 kDa nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.

Database

Research Area

DATA IMAGES
GTX70232_40004_20151112_WB_Fraction_18122101_798.jpg

GTX70232 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] (GTX70232) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70232_40004_ChIP_18122101_183.jpg

GTX70232 ChIP assay Image

RbAp48 antibody immunoprecipitates RbAp48 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune mouse IgG B. 5 μg of RbAp48 antibody (GTX70232) The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.

RbAp48-antibody-11G10-GTX70232-IHC-1_18122101_259.jpg

GTX70232 IHC Image


RbAp48-antibody-11G10-GTX70232-ICC_IF-1_18122101_485.jpg

GTX70232 ICC/IF Image

Immunofluorescence analysis of HeLa, using RbAp48(GTX70232) antibody at 1:100 dilution.

GTX70232_40004_20170713_WB_Fraction_18122101_975.jpg

GTX70232 WB Image

Orthogonal

SH-SY5Y whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] (GTX70232) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
Application Reference