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RbAp48 antibody [13D10]

Anti-RbAp48 antibody [13D10] used in Western Blot (WB). GTX70234
Anti-RbAp48 antibody [13D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70234
Anti-RbAp48 antibody [13D10] used in Western Blot (WB). GTX70234
Anti-RbAp48 antibody [13D10] used in Immunoprecipitation (IP). GTX70234

Cat No. GTX70234

Host

Mouse

Clonality

Monoclonal

Clone Name

13D10

Isotype

IgG2b

Application

WB, ICC/IF, IP, IHC

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa nuclear extract , SH-SY-5Y , SH-SY-5Y nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rb Binding Protein 4, Chromatin Remodeling Factor , Nurf55 , Rbap48 , Lin-53 , Rbbp4

Cellular Localization

Nuclear

Background

RbAp48-13D10 recognizes full-length RbAp48, a 48a nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.

Database

Research Area

DATA IMAGES

Anti-RbAp48 antibody [13D10] used in Western Blot (WB). GTX70234

GTX70234 WB Image

Detection of RbAp48 in HeLa nuclear extract (GTX24105)

Anti-RbAp48 antibody [13D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70234

GTX70234 ICC/IF Image

RbAp48 antibody [13D10] detects RbAp48 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RbAp48 protein stained by RbAp48 antibody [13D10] (GTX70234) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-RbAp48 antibody [13D10] used in Western Blot (WB). GTX70234

GTX70234 WB Image

SH-SY-5Y whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [13D10] (GTX70234) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-RbAp48 antibody [13D10] used in Immunoprecipitation (IP). GTX70234

GTX70234 IP Image

Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 μg of RbAp48 antibody [13D10] (GTX70234).
Western blot analysis was performed using RbAp48 antibody [13D10] (GTX70234).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329