SH-SY5Y whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [C1C3] (GTX114707) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Huh7 xenograft, using RbAp48(GTX114707) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX114707 diluted at 1:1000