GeneTex
United States (US)

RbAp48 antibody [N1C2]

Orthogonal
GTX115554_40219_IHC_18121410_558.jpg
GTX115554_40219_20150410_IFA_2_18121410_657.jpg
GTX115554_40219_20170720_WB_Fraction_18121410_791.jpg
GTX115554_WB_18121410_248.jpg
GTX115554_40219_20150410_IFA_18121410_750.jpg

Cat No. GTX115554

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , SH-SY5Y , SH-SY5Y nuclear extract

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RbAp48. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rb Binding Protein 4, Chromatin Remodeling Factor,Nurf55,Rbap48,Lin-53,Rbbp4

Background

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115554_40219_IHC_18121410_558.jpg

GTX115554 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using RbAp48(GTX115554) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115554_40219_20150410_IFA_2_18121410_657.jpg

GTX115554 ICC/IF Image

RbAp48 antibody [N1C2] detects RbAp48 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RbAp48 protein stained by RbAp48 antibody [N1C2] (GTX115554) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX115554_40219_20170720_WB_Fraction_18121410_791.jpg

GTX115554 WB Image

Orthogonal

SH-SY5Y whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [N1C2] (GTX115554) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115554_WB_18121410_248.jpg

GTX115554 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
10% SDS PAGE
GTX115554 diluted at 1:3000

GTX115554_40219_20150410_IFA_18121410_750.jpg

GTX115554 ICC/IF Image

RbAp48 antibody [N1C2] detects RbAp48 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RbAp48 protein stained by RbAp48 antibody [N1C2] (GTX115554) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf