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RbAp48 antibody [N3C3]

Orthogonal
Anti-RbAp48 antibody [N3C3] used in Western Blot (WB). GTX115555
Anti-RbAp48 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115555
Anti-RbAp48 antibody [N3C3] used in Western Blot (WB). GTX115555
Anti-RbAp48 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115555
Anti-RbAp48 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX115555

Cat. No. GTX115555

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all RbAp48 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

A431 , SH-SY5Y nuclear extract

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RbAp48. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10625508

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB binding protein 4, chromatin remodeling factor , NURF55 , RBAP48 , lin-53

Background

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RbAp48 antibody [N3C3] used in Western Blot (WB). GTX115555

GTX115555 WB Image

Orthogonal

SH-SY5Y whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [N3C3] (GTX115555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RbAp48 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115555

GTX115555 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB231 xenograft, using RbAp48(GTX115555) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RbAp48 antibody [N3C3] used in Western Blot (WB). GTX115555

GTX115555 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX115555 diluted at 1:5000

Anti-RbAp48 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115555

GTX115555 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using RbAp48(GTX115555) antibody at 1:200 dilution.

Anti-RbAp48 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX115555

GTX115555 IHC-Wm Image

RbAp48 antibody [N3C3] detects RbAp48 protein on whole-mount zebrafish embryos by immunohistochemical analysis.?
Sample: Paraformaldehyde-fixed zebrafish embryos.
RbAp48 antibody [N3C3] (GTX115555) dilution: 1:200.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169