*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A431 , Jurkat , Raji , SH-SY5Y , SH-SY5Y nuclear extract ,
Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)
Recombinant protein encompassing a sequence within the center region of human RbAp48. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
NURF55 antibody, RBAP48 antibody, RBBP4 antibody, CAF-I p48 antibody, CAF-I 48 kDa subunit antibody, RBBP-4 antibody, chromatin assembly factor I p48 subunit antibody, nucleosome-remodeling factor subunit RBAP48 antibody, chromatin assembly factor/CAF-1 p48 subunit antibody, retinoblastoma-binding protein 4 antibody, CAF-1 subunit C antibody, histone-binding protein RBBP4 antibody, retinoblastoma-binding protein p48 antibody, MSI1 protein homolog antibody, chromatin assembly factor 1 subunit C antibody, retinoblastoma binding protein 4 antibody
This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
GTX115555 WB Image
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX115555 diluted at 1:5000
GTX115555 IHC-P Image
Immunohistochemical analysis of paraffin-embedded MDAMB231 xenograft, using RbAp48(GTX115555) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX115555 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A431, using RbAp48(GTX115555) antibody at 1:200 dilution.
GTX115555 WB Image
SH-SY5Y whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [N3C3] (GTX115555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.