GeneTex
United States (US)

RbAp48 antibody [N3C3]

Orthogonal
GTX115555_WB_18121410_928.jpg
GTX115555_40219_IHC_18121410_942.jpg
GTX115555_40219_IFA_18121410_384.jpg
GTX115555_40219_20170720_WB_Fraction_18121410_964.jpg

Cat No. GTX115555

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

A431 , Jurkat , Raji , SH-SY5Y , SH-SY5Y nuclear extract ,

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.65mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RbAp48. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NURF55 antibody, RBAP48 antibody, RBBP4 antibody, CAF-I p48 antibody, CAF-I 48 kDa subunit antibody, RBBP-4 antibody, chromatin assembly factor I p48 subunit antibody, nucleosome-remodeling factor subunit RBAP48 antibody, chromatin assembly factor/CAF-1 p48 subunit antibody, retinoblastoma-binding protein 4 antibody, CAF-1 subunit C antibody, histone-binding protein RBBP4 antibody, retinoblastoma-binding protein p48 antibody, MSI1 protein homolog antibody, chromatin assembly factor 1 subunit C antibody, retinoblastoma binding protein 4 antibody

Background

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX115555_WB_18121410_928.jpg

GTX115555 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX115555 diluted at 1:5000

GTX115555_40219_IHC_18121410_942.jpg

GTX115555 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB231 xenograft, using RbAp48(GTX115555) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115555_40219_IFA_18121410_384.jpg

GTX115555 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using RbAp48(GTX115555) antibody at 1:200 dilution.

GTX115555_40219_20170720_WB_Fraction_18121410_964.jpg

GTX115555 WB Image

Orthogonal

SH-SY5Y whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [N3C3] (GTX115555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf