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RecA antibody

Anti-RecA antibody used in Western Blot (WB). GTX64150
Anti-RecA antibody used in Immunoprecipitation (IP). GTX64150

Cat No. GTX64150

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP, ELISA

Reactivity

E. coli
Package
50 μg ($319)

APPLICATION

Application Note

Western blotting (1:3,000 dilution), Immunoprecipitation (1:600 dilution)

PROPERTIES

Form

Liquid

Buffer

Antibody solution at 1.0 mg/ml in PBS with 50% glycerol, filter-sterilized

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

Highly purified full-length recombinant E. coli RecA protein

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-RecA antibody used in Western Blot (WB). GTX64150

GTX64150 WB Image

Western blot analysis of RecA protein in crude extract of E. coli Lane 1, Purified RecA 8 ng. Lane 2, Wild type. Lane 3, ⊿recA mutant. Lane 4, ⊿lexA mutant (SOS genes overexpressed). Antibody used at 1/3,000 dilution. Cell extracts, 2 μg

Anti-RecA antibody used in Immunoprecipitation (IP). GTX64150

GTX64150 IP Image

Immunoprecipitation of RecA protein from crude extract of E. coli cells. Antibody 2 μg was adsorbed to proteinA magnetic beads to precipitate RecA protein from supernatant of sonic disrupted E. coli cells. The precipitate was analyzed by western blot.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 319