GeneTex
United States (US)

RecA antibody

GTX64150_20170328_WB_18121411_314.jpg
GTX64150_20170328_IP_18121411_543.jpg

Cat No. GTX64150

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, ELISA
Reactivity E. coli
APPLICATION

Application Note

Western blotting (1:3,000 dilution), Immunoprecipitation (1:600 dilution)
PROPERTIES

Form

Liquid

Buffer

Antibody solution at 1.0 mg/ml in PBS with 50% glycerol, filter-sterilized

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

E. coli

Immunogen

Highly purified full-length recombinant E. coli RecA protein

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX64150_20170328_WB_18121411_314.jpg

GTX64150 WB Image

Western blot analysis of RecA protein in crude extract of E. coli Lane 1, Purified RecA 8 ng. Lane 2, Wild type. Lane 3, ⊿recA mutant. Lane 4, ⊿lexA mutant (SOS genes overexpressed). Antibody used at 1/3,000 dilution. Cell extracts, 2 μg

GTX64150_20170328_IP_18121411_543.jpg

GTX64150 IP Image

Immunoprecipitation of RecA protein from crude extract of E. coli cells. Antibody 2 μg was adsorbed to proteinA magnetic beads to precipitate RecA protein from supernatant of sonic disrupted E. coli cells. The precipitate was analyzed by western blot.

Package List Price ($)
$ 319