GeneTex
United States (US)

RecQ1 antibody [C1C3]

GTX100085_IHC_19011506_971.jpg
GTX100085_WB_M_19011506_285.jpg
GTX100085_WB_19011506_789.jpg
GTX100085_40107_20150529_IP_19011506_427.jpg
GTX100085_40107_20150506_IFA_19011506_360.jpg

Cat No. GTX100085

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RecQ1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Recq Like Helicase,Recql1,Recq1,Recql

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability. The biological function of this helicase has not yet been determined. Two alternatively spliced transcripts, which encode the same isoform but differ in their 5' and 3' UTRs, have been described. [provided by RefSeq]

Database

DATA IMAGES
GTX100085_IHC_19011506_971.jpg

GTX100085 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RecQ1(GTX100085) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100085_WB_M_19011506_285.jpg

GTX100085 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX100085 diluted at 1:1000

GTX100085_WB_19011506_789.jpg

GTX100085 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX100085 diluted at 1:1000

GTX100085_40107_20150529_IP_19011506_427.jpg

GTX100085 IP Image

Immunoprecipitation of RecQ1 protein from HeLa whole cell extracts using 5 μg of RecQ1 antibody [C1C3] (GTX100085).
Western blot analysis was performed using RecQ1 antibody [C1C3] (GTX100085).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100085_40107_20150506_IFA_19011506_360.jpg

GTX100085 ICC/IF Image

RecQ1 antibody [C1C3] detects RecQ1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RecQ1 protein stained by RecQ1 antibody [C1C3] (GTX100085) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 8 μm.

SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169