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RecQ1 antibody [N1N2], N-term

Anti-RecQ1 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX100084
Anti-RecQ1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100084
Anti-RecQ1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100084
Anti-RecQ1 antibody [N1N2], N-term used in Western Blot (WB). GTX100084

Cat. No. GTX100084

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RecQ1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

A431 , HeLa , A375

Predict Reactivity

Mouse, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RecQ1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241252

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RecQ like helicase , RECQL1 , RecQ1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability. The biological function of this helicase has not yet been determined. Two alternatively spliced transcripts, which encode the same isoform but differ in their 5' and 3' UTRs, have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RecQ1 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX100084

GTX100084 IP Image

Immunoprecipitation of RecQ1 protein from HeLa whole cell extracts using 5 μg of RecQ1 antibody [N1N2], N-term (GTX100084).
Western blot analysis was performed using RecQ1 antibody [N1N2], N-term (GTX100084).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-RecQ1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100084

GTX100084 ICC/IF Image

RecQ1 antibody [N1N2], N-term detects RecQ1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RecQ1 protein stained by RecQ1 antibody [N1N2], N-term (GTX100084) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-RecQ1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100084

GTX100084 IHC-P Image

RecQ1 antibody [N1N2], N-term detects RecQ1 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RecQ1 antibody [N1N2], N-term (GTX100084) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RecQ1 antibody [N1N2], N-term used in Western Blot (WB). GTX100084

GTX100084 WB Image

RecQ1 antibody [N1N2], N-term detects RecQ1 protein by Western blot analysis.
A. 30 μg A431 whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg A375 whole cell lysate/extract
7.5 % SDS-PAGE
RecQ1 antibody [N1N2], N-term (GTX100084) dilution: 1:1000

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169