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Reelin antibody

Anti-Reelin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133226
Anti-Reelin antibody used in Western Blot (WB). GTX133226
Anti-Reelin antibody used in IHC (Frozen sections) (IHC-Fr). GTX133226

Cat No. GTX133226

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Reelin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

387 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Sheep, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

7.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse Reelin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

reelin , reeler , rl

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and ApoER2 induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation.

Database

Research Area

DATA IMAGES

Anti-Reelin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133226

GTX133226 ICC/IF Image

Reelin antibody detects Reelin protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Reelin protein stained by Reelin antibody (GTX133226) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Reelin antibody used in Western Blot (WB). GTX133226

GTX133226 WB Image

Various tissue extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Reelin antibody (GTX133226) diluted at 1:1000.

Anti-Reelin antibody used in IHC (Frozen sections) (IHC-Fr). GTX133226

GTX133226 IHC-Fr Image

Reelin antibody detects Reelin protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebral cortex.
Green: Reelin stained by Reelin antibody (GTX133226) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169