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RelB antibody [HL2222]

Anti-RelB antibody [HL2222] used in Western Blot (WB). GTX638267
Anti-RelB antibody [HL2222] used in Western Blot (WB). GTX638267
Anti-RelB antibody [HL2222] used in Western Blot (WB). GTX638267
Anti-RelB antibody [HL2222] used in IHC (Paraffin sections) (IHC-P). GTX638267
Anti-RelB antibody [HL2222] used in Western Blot (WB). GTX638267

Cat. No. GTX638267

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2222

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RelB. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RELB proto-oncogene, NF-kB subunit , I-REL , IMD53 , IREL , REL-B

Cellular Localization

Nucleus,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Background

Enables mRNA N6-methyladenosine dioxygenase activity. Involved in RNA metabolic process; mRNA export from nucleus; and response to hypoxia. Located in Golgi apparatus; cytosol; and nuclear speck. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-RelB antibody [HL2222] used in Western Blot (WB). GTX638267

GTX638267 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (GTX638267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-RelB antibody [HL2222] used in Western Blot (WB). GTX638267

GTX638267 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (GTX638267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RelB antibody [HL2222] used in Western Blot (WB). GTX638267

GTX638267 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (GTX638267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-RelB antibody [HL2222] used in IHC (Paraffin sections) (IHC-P). GTX638267

GTX638267 IHC-P Image

RelB antibody [HL2222] detects RelB protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
RelB stained by RelB antibody [HL2222] (GTX638267) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RelB antibody [HL2222] used in Western Blot (WB). GTX638267

GTX638267 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (GTX638267) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations