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RelB antibody

Overexpression IP/MS
GTX102333_40758_IP_19011506_534.jpg
GTX102333_40758_20150320_IHC_19011506_490.jpg
GTX102333_40758_ChIP_19011506_947.jpg
GTX102333_43005_20180817_WB_B_19011506_540.jpg
GTX102333_43005_20171110_WB_19011506_931.jpg
GTX102333_40758_WB_M_19011506_207.jpg

Cat No. GTX102333

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP, ChIP assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

Raji , NCI-H929 , NIH-3T3 , RelB-transfected 293T

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377382)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RelB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

I-REL antibody, IREL antibody, REL-B antibody, RELB antibody, v-rel reticuloendotheliosis viral oncogene homolog B, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody, v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3) antibody, transcription factor RelB antibody, v-rel reticuloendotheliosis viral oncogene homolog B antibody

Cellular Localization

Nucleus

Background

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.

Database

Research Area

DATA IMAGES
GTX102333_40758_IP_19011506_534.jpg

GTX102333 IP Image

RelB antibody immunoprecipitates RelB protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of RelB protein by 2 μg of RelB antibody (GTX102333) 7.5% SDS-PAGE The immunoprecipitated RelB protein was detected by RelB antibody (GTX102333) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX102333_40758_20150320_IHC_19011506_490.jpg

GTX102333 IHC-P Image

RelB antibody detects RelB protein at cytoplasm on human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
RelB antibody (GTX102333) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102333_40758_ChIP_19011506_947.jpg

GTX102333 ChIP assay Image

Cross-linked ChIP was performed with THP-1 chromatin extract treated with LPS (1.0 μg/ml for 3 h) and 5 μg of either normal rabbit IgG or anti-RelB antibody. The precipitated DNA was detected by PCR with primer set targeting to IL-1βpromoter.
ChIP experiment and primer designs are based on J Biol Chem. 2009 Oct 9;284(41):27857-65.

GTX102333_43005_20180817_WB_B_19011506_540.jpg

GTX102333 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody (GTX102333) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102333_43005_20171110_WB_19011506_931.jpg

GTX102333 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody (GTX102333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102333_40758_WB_M_19011506_207.jpg

GTX102333 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX102333 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169