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RelB antibody

Anti-RelB antibody used in Immunoprecipitation (IP). GTX102333
Anti-RelB antibody used in IHC (Paraffin sections) (IHC-P). GTX102333
Anti-RelB antibody used in ChIP assay (ChIP assay). GTX102333
Anti-RelB antibody used in Western Blot (WB). GTX102333
Anti-RelB antibody used in Western Blot (WB). GTX102333
Anti-RelB antibody used in Western Blot (WB). GTX102333

Cat No. GTX102333

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP, ChIP assay

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

Raji , NCI-H929 , NIH-3T3 , RelB-transfected 293T

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377382)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RelB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RELB proto-oncogene, NF-kB subunit , I-REL , IMD53 , IREL , REL-B

Cellular Localization

Nucleus

Background

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.

Database

Research Area

DATA IMAGES

Anti-RelB antibody used in Immunoprecipitation (IP). GTX102333

GTX102333 IP Image

RelB antibody immunoprecipitates RelB protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of RelB protein by 2 μg of RelB antibody (GTX102333) 7.5% SDS-PAGE The immunoprecipitated RelB protein was detected by RelB antibody (GTX102333) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-RelB antibody used in IHC (Paraffin sections) (IHC-P). GTX102333

GTX102333 IHC-P Image

RelB antibody detects RelB protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RelB stained by RelB antibody (GTX102333) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RelB antibody used in ChIP assay (ChIP assay). GTX102333

GTX102333 ChIP assay Image

Cross-linked ChIP was performed with THP-1 chromatin extract treated with LPS (1.0 μg/ml for 3 h) and 5 μg of either normal rabbit IgG or anti-RelB antibody. The precipitated DNA was detected by PCR with primer set targeting to IL-1βpromoter.
ChIP experiment and primer designs are based on J Biol Chem. 2009 Oct 9;284(41):27857-65.

Anti-RelB antibody used in Western Blot (WB). GTX102333

GTX102333 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody (GTX102333) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RelB antibody used in Western Blot (WB). GTX102333

GTX102333 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody (GTX102333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RelB antibody used in Western Blot (WB). GTX102333

GTX102333 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX102333 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf