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Renilla Luciferase antibody

Anti-Renilla Luciferase antibody used in Western Blot (WB). GTX125851
Anti-Renilla Luciferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125851

Cat. No. GTX125851

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Wm
Package
100 μl ($399),
25 μl ($169)
See all Renilla Luciferase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T transfected Renilla Luciferase

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.14 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length Renilla Luciferase recombinant protein

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176824

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Renilla Luciferase , Luciferase

Research Area

DATA IMAGES

Anti-Renilla Luciferase antibody used in Western Blot (WB). GTX125851

GTX125851 WB Image

Renilla Luciferase antibody detects Renilla Luciferase protein by Western blot analysis. Non-transfected (-) and Renilla Luciferase -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Renilla Luciferase antibody (GTX125851) at a dilution of 1:2000.

Anti-Renilla Luciferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125851

GTX125851 ICC/IF Image

Renilla Luciferase antibody detects Renilla Luciferase protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Renilla Luciferase stained by Renilla Luciferase antibody (GTX125851) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

C Coubris et al. PLoS One 2024; 19 (3) : e0298185 A brittle star is born: Ontogeny of luminous capabilities in Amphiura filiformis
Treffers EE et al. PLoS Pathog 2023; 19 (2) : e1011179 The alphavirus nonstructural protein 2 NTPase induces a host translational shut-off through phosphorylation of eEF2 via cAMP-PKA-eEF2K signaling.
Application : WB
Tessler M et al. Sci Rep 2020; 10 (1) : 17724 A putative chordate luciferase from a cosmopolitan tunicate indicates convergent bioluminescence evolution across phyla.
Application : ICC/IF
den Hoed J et al. Sci Rep 2018; 8 (1) : 14279 Functional characterization of TBR1 variants in neurodevelopmental disorder.
Application : WB
Gangadaran P et al. Oncotarget 2017; 8 (66) : 109894-109914 A new bioluminescent reporter system to study the biodistribution of systematically injected tumor-derived bioluminescent extracellular vesicles in mice.
Application : WB
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Application Reference
C Coubris et al. PLoS One 2024; 19 (3):e0298185 A brittle star is born: Ontogeny of luminous capabilities in Amphiura filiformis
Treffers EE et al. PLoS Pathog 2023; 19 (2):e1011179 The alphavirus nonstructural protein 2 NTPase induces a host translational shut-off through phosphorylation of eEF2 via cAMP-PKA-eEF2K signaling.
Application : WB
Tessler M et al. Sci Rep 2020; 10 (1):17724 A putative chordate luciferase from a cosmopolitan tunicate indicates convergent bioluminescence evolution across phyla.
Application : ICC/IF
den Hoed J et al. Sci Rep 2018; 8 (1):14279 Functional characterization of TBR1 variants in neurodevelopmental disorder.
Application : WB
Gangadaran P et al. Oncotarget 2017; 8 (66):109894-109914 A new bioluminescent reporter system to study the biodistribution of systematically injected tumor-derived bioluminescent extracellular vesicles in mice.
Application : WB
Delroisse J et al. Zool. Anz. 2017; 269 (2017):43112 Fine structure of the luminous spines and luciferase detection in the brittle star Amphiura filiformis.
Application : IHC-Wm
Delroisse J et al. Open Biol 2017; 7 (4) A puzzling homology: a brittle star using a putative cnidarian-type luciferase for bioluminescence.
Pecot J et al. Cell Rep 2016; 17 (12):3347-3358 Tight Sequestration of BH3 Proteins by BCL-xL at Subcellular Membranes Contributes to Apoptotic Resistance.
Masuho I et al. J Biol Chem 2013; 288 (35):25129-42 Macromolecular composition dictates receptor and G protein selectivity of regulator of G protein signaling (RGS) 7 and 9-2 protein complexes in living cells.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169