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Renin antibody [N2C2], Internal

Anti-Renin antibody [N2C2], Internal used in Western Blot (WB). GTX105734
Anti-Renin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105734
Anti-Renin antibody [N2C2], Internal used in Western Blot (WB). GTX105734

Cat No. GTX105734

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Renin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

renin , HNFJ2

Cellular Localization

Secreted , Membrane

Background

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Renin antibody [N2C2], Internal used in Western Blot (WB). GTX105734

GTX105734 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Renin antibody [N2C2], Internal (GTX105734) diluted at 1:500.

Anti-Renin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105734

GTX105734 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using Renin(GTX105734) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Renin antibody [N2C2], Internal used in Western Blot (WB). GTX105734

GTX105734 WB Image

Renin antibody [N2C2], Internal detects REN protein by Western blot analysis.
A. 30 µg K562 whole cell lysate/extract
10 % SDS-PAGE
Renin antibody [N2C2], Internal (GTX105734) dilution: 1:1000

REFERENCE
There are currently no references for Renin antibody [N2C2], Internal (GTX105734). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Renin antibody [N2C2], Internal (GTX105734). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf