GeneTex
United States (US)

Renin antibody [N2C2], Internal

GTX105734_39946_20151119_WB_Merged_19011506_852.jpg
GTX105734_39946_IHC_19011506_640.jpg
GTX105734_39946_20160127_IFA_19011506_422.jpg
GTX105734_39946_WB_19011506_299.jpg

Cat No. GTX105734

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Renin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ10761 antibody, HNFJ2 antibody, REN antibody, renin precursor, renal antibody, angiotensin-forming enzyme antibody, renin antibody, angiotensinogenase antibody

Cellular Localization

Secreted , Membrane

Background

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105734_39946_20151119_WB_Merged_19011506_852.jpg

GTX105734 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Renin antibody [N2C2], Internal (GTX105734) diluted at 1:500.

GTX105734_39946_IHC_19011506_640.jpg

GTX105734 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using Renin(GTX105734) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105734_39946_20160127_IFA_19011506_422.jpg

GTX105734 ICC/IF Image

Renin antibody [N2C2], Internal detects Renin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Renin protein stained by Renin antibody [N2C2], Internal (GTX105734) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX105734_39946_WB_19011506_299.jpg

GTX105734 WB Image

Renin antibody [N2C2], Internal detects REN protein by Western blot analysis.
A. 30 µg K562 whole cell lysate/extract
10 % SDS-PAGE
Renin antibody [N2C2], Internal (GTX105734) dilution: 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169