GeneTex
United States (US)

Rent2 antibody [C3], C-term

GTX107694_40058_IFA_19011507_892.jpg
GTX107694_40058_WB_M_Brain_19011507_357.jpg

Cat No. GTX107694

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

148 kDa. ( Note )

Positive Control

mouse brain

Predict Reactivity

Rat, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Rent2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Upf2, Regulator Of Nonsense Mediated Mrna Decay,Hupf2,Rent2,Smg-3,Upf2

Cellular Localization

Cytoplasm , perinuclear region

Background

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107694_40058_IFA_19011507_892.jpg

GTX107694 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Rent2(GTX107694) antibody at 1:500 dilution.

GTX107694_40058_WB_M_Brain_19011507_357.jpg

GTX107694 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
5% SDS PAGE
GTX107694 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169