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Respiratory Syncytial virus Nucleoprotein antibody [HL1247]

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in Western Blot (WB). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in Lateral Flow (Lateral Flow). GTX636649
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in Western Blot (WB). GTX636649

Cat. No. GTX636649

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1247

Isotype

IgG

Application

WB, IP, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

Respiratory syncytial virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IP Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX636649, Detection : GTX636648 or Capture : GTX636648 / GTX636650, Detection : GTX636649.
Please notice that GTX636649 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636648 / GTX636650. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Respiratory syncytial virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Respiratory Syncytial virus Nucleoprotein. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910028

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649

GTX636649 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649

GTX636649 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus nucleoprotein using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 μg/mL)
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649

GTX636649 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649

GTX636649 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture:Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP
Pair 2:
Capture:Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP
Pair 3:
Capture:Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649

GTX636649 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649

GTX636649 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649

GTX636649 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649

GTX636649 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) (84.76-1.32 nM). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (1 μg/mL) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649

GTX636649 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory syncytial virus type A Nucleoprotein, DDDDK tag (GTX136751-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649

GTX636649 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag (GTX136931-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649

GTX636649 ELISA Image

Sandwich ELISA of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in ELISA (ELISA). GTX636649

GTX636649 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant E.coli expressed, C-terminal respiratory syncytial virus type A nucleoprotein (50 ng). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (160000-1.9*10-2 pM) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in Western Blot (WB). GTX636649

GTX636649 WB Image

Respiratory syncytial virus lysate (1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in Lateral Flow (Lateral Flow). GTX636649

GTX636649 Lateral Flow Image

Detection of respiratory syncytial virus type A nucleoprotein (GTX136751-pro) (0.5 μg/ml) by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1247] used in Western Blot (WB). GTX636649

GTX636649 WB Image

Respiratory syncytial virus lysates (1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

Respiratory Syncytial virus Nucleoprotein antibody [HL1247] Cat. No. GTX636649

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-Jul-2022
Score :
Application Tested : IP
Sample Species : RSV
Sample : HepG2 cells with/without RSV (A Long Strain) infection, MOI 1, 24hrs
Figure Note :
The image is from a collaborating laboratory.
Date : Anonymous submitted on 10-Jun-2022
Score :
Application Tested : WB
Sample Species : RSV
Sample : HepG2 cells with/without RSV (A Long Strain) infection, MOI 1, 24hrs
Amount used : 20μg
Blocking : 5% milk in PBS-Tween 0.02%
Lane Description : Dilution : 1:1000
Figure Note :
The image is from a collaborating laboratory.
Package List Price ($)
$ 399
$ 169
Custom Conjugations