Respiratory Syncytial virus Nucleoprotein antibody [HL1248]

Name: Respiratory Syncytial virus Nucleoprotein antibody [HL1248]
Brand: GeneTex
SKU: GTX636650
Price: 399.00 USD
Rating: 5 (3 reviews)

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636650

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Western Blot (WB). GTX636650

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Lateral Flow (Lateral Flow). GTX636650

Cat. No. GTX636650

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1248
Isotype	IgG
Application	WB, ICC/IF, IP, ELISA, Lateral Flow, Sandwich ELISA
Reactivity	Respiratory syncytial virus
Package	100 μl ($399), 25 μl ($169)

See all Respiratory Syncytial virus Nucleoprotein products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
IP	Assay dependent
ELISA	Assay dependent
Lateral Flow	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX636650, Detection : GTX636648 / GTX636649 or Capture : GTX636648, Detection : GTX636650. Please notice that GTX636650 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636648. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Respiratory syncytial virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Respiratory Syncytial virus Nucleoprotein. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910029

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

GTX636650 ELISA Image

Sandwich ELISA of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

GTX636650 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant E.coli expressed, C-terminal respiratory syncytial virus type A nucleoprotein (50 ng). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (160000-1.9*10^-2 pM) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)

GTX636650 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) (84.76-1.32 nM). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (1 μg/mL) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus nucleoprotein using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 μg/mL)
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

GTX636650 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory syncytial virus type A Nucleoprotein, DDDDK tag (GTX136751-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

GTX636650 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag (GTX136931-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

GTX636650 ICC/IF Image

Respiratory Syncytial virus Nucleoprotein antibody [HL1248] detects Respiratory Syncytial virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Respiratory Syncytial virus Nucleoprotein stained by Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636650 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636650 Lateral Flow Image

Detection of Respiratory Syncytial virus Nucleoprotein by lateral flow assay using the monoclonal antibody pair.

Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) [HL1248])
Detection: Respiratory Syncytial virus Nucleoprotein antibody (GTX636648) [HL1246])

Samples (25 ng) :
1. RSV type A Nucleoprotein (GTX136751-pro)
2. RSV type B Nucleoprotein (GTX136931-pro)
3. SARS-CoV-2 Nucleocapsid protein (GTX135592-pro)
4. FluA (H1N1) Nucleoprotein (GTX135904-pro)
5. FluA (H3N2) Nucleoprotein (GTX135903-pro)
6. FluB Nucleoprotein (GTX136277-pro)
7. Lysis buffer

GTX636650 WB Image

Non-infected (–) and infected (+) HEp-2 whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636650 Lateral Flow Image

Detection of respiratory syncytial virus type A nucleoprotein (GTX136751-pro) (0.5 μg/ml) by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

REFERENCE

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REVIEW

Respiratory Syncytial virus Nucleoprotein antibody [HL1248] Cat. No. GTX636650

Rating

( Average 5 based on 3 users reviews)

Application

Western Blot(WB)		( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)		( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)		( Average 5 based on 1 users reviews)

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Immunoprecipitation (IP). GTX636650

Date : Anonymous submitted on 13-Jul-2022

Score :

Application Tested : IP

Sample Species : RSV

Sample : HepG2 cells with/without RSV (A Long Strain) infection, MOI 1, 24hrs

Figure Note :
The image is from a collaborating laboratory.

Date : Anonymous submitted on 10-Jun-2022

Score :

Application Tested : ICC/IF

Sample Species : RSV

Sample : HepG2 cells with/without mCherry-RSV (A Long Strain) infection, MOI 1, 24hrs

Fixation : 4% PFA

Permeabilization /Antigen retrieval : 3% BSA and 0.1% Triton in PBS

Lane Description :
Green : Primary antibody
Dilution : 1:1000

Figure Note :
The image is from a collaborating laboratory.

Date : Anonymous submitted on 10-Jun-2022

Score :

Application Tested : WB

Sample Species : RSV

Sample : HepG2 cells with/without RSV (A Long Strain) infection, MOI 1, 24hrs

Amount used : 20μg

Blocking : 5% milk in PBS-Tween 0.02%

Lane Description : Dilution : 1:1000

Figure Note :
The image is from a collaborating laboratory.

Submit a Review

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty Custom Conjugations

Respiratory Syncytial virus Nucleoprotein antibody [HL1248]

Cat. No. GTX636650

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Research Area

DATA IMAGES

GTX636650 ELISA Image

GTX636650 ELISA Image

GTX636650 ELISA Image

GTX636650 ELISA Image

GTX636650 ELISA Image

GTX636650 ELISA Image

GTX636650 ELISA Image

GTX636650 ELISA Image

GTX636650 ELISA Image

GTX636650 ELISA Image

GTX636650 ELISA Image

GTX636650 ICC/IF Image

GTX636650 WB Image

GTX636650 Lateral Flow Image

GTX636650 WB Image

GTX636650 Lateral Flow Image

REFERENCE

REVIEW

Respiratory Syncytial virus Nucleoprotein antibody [HL1248] Cat. No. GTX636650

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.