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Respiratory Syncytial virus Nucleoprotein antibody [HL1248]

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Western Blot (WB). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Lateral Flow (Lateral Flow). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Western Blot (WB). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Lateral Flow (Lateral Flow). GTX636650

Cat. No. GTX636650

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1248

Isotype

IgG

Application

WB, ICC/IF, IP, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

Respiratory syncytial virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX636650, Detection : GTX636648 / GTX636649 or Capture : GTX636648, Detection : GTX636650.
Please notice that GTX636650 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636648. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Respiratory syncytial virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Respiratory Syncytial virus Nucleoprotein. (strain A2)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910029

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant E.coli expressed, C-terminal respiratory syncytial virus type A nucleoprotein (50 ng). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (160000-1.9*10-2 pM) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) (84.76-1.32 nM). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (1 μg/mL) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus nucleoprotein using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 μg/mL)
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory syncytial virus type A Nucleoprotein, DDDDK tag (GTX136751-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag (GTX136931-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636650

GTX636650 ICC/IF Image

Respiratory Syncytial virus Nucleoprotein antibody [HL1248] detects Respiratory Syncytial virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Respiratory Syncytial virus Nucleoprotein stained by Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Western Blot (WB). GTX636650

GTX636650 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Lateral Flow (Lateral Flow). GTX636650

GTX636650 Lateral Flow Image

Detection of respiratory syncytial virus type A nucleoprotein (GTX136751-pro) (0.5 μg/ml) by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Western Blot (WB). GTX636650

GTX636650 WB Image

Non-infected (–) and infected (+) HEp-2 whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Lateral Flow (Lateral Flow). GTX636650

GTX636650 Lateral Flow Image

Detection of Respiratory Syncytial virus Nucleoprotein by lateral flow assay using the monoclonal antibody pair.

Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) [HL1248])
Detection: Respiratory Syncytial virus Nucleoprotein antibody (GTX636648) [HL1246])

Samples (25 ng) :
1. RSV type A Nucleoprotein (GTX136751-pro)
2. RSV type B Nucleoprotein (GTX136931-pro)
3. SARS-CoV-2 Nucleocapsid protein (GTX135592-pro)
4. FluA (H1N1) Nucleoprotein (GTX135904-pro)
5. FluA (H3N2) Nucleoprotein (GTX135903-pro)
6. FluB Nucleoprotein (GTX136277-pro)
7. Lysis buffer

REFERENCE

There are currently no references for Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650). Be the first to share your publications with this product.

REVIEW

Respiratory Syncytial virus Nucleoprotein antibody [HL1248] Cat. No. GTX636650

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-Jul-2022
Score :
Application Tested : IP
Sample Species : RSV
Sample : HepG2 cells with/without RSV (A Long Strain) infection, MOI 1, 24hrs
Figure Note :
The image is from a collaborating laboratory.
Date : Anonymous submitted on 10-Jun-2022
Score :
Application Tested : ICC/IF
Sample Species : RSV
Sample : HepG2 cells with/without mCherry-RSV (A Long Strain) infection, MOI 1, 24hrs
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 3% BSA and 0.1% Triton in PBS
Lane Description :
Green : Primary antibody
Dilution : 1:1000
Figure Note :
The image is from a collaborating laboratory.
Date : Anonymous submitted on 10-Jun-2022
Score :
Application Tested : WB
Sample Species : RSV
Sample : HepG2 cells with/without RSV (A Long Strain) infection, MOI 1, 24hrs
Amount used : 20μg
Blocking : 5% milk in PBS-Tween 0.02%
Lane Description : Dilution : 1:1000
Figure Note :
The image is from a collaborating laboratory.
Package List Price ($)
$ 399
$ 169
Custom Conjugations