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Respiratory Syncytial virus Nucleoprotein antibody [HL1248]

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Western Blot (WB). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Western Blot (WB). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Lateral Flow (Lateral Flow). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636650
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Lateral Flow (Lateral Flow). GTX636650

Cat. No. GTX636650

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1248

Isotype

IgG

Application

WB, ICC/IF, IP, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

Respiratory syncytial virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX636650, Detection : GTX636648 / GTX636649 or Capture : GTX636648, Detection : GTX636650.
Please notice that GTX636650 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636648. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Respiratory syncytial virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Respiratory Syncytial virus Nucleoprotein. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910029

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant E.coli expressed, C-terminal respiratory syncytial virus type A nucleoprotein (50 ng). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (160000-1.9*10-2 pM) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) (84.76-1.32 nM). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (1 μg/mL) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory syncytial virus type A Nucleoprotein, DDDDK tag (GTX136751-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag (GTX136931-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in ELISA (ELISA). GTX636650

GTX636650 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus nucleoprotein using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 μg/mL)
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Western Blot (WB). GTX636650

GTX636650 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Western Blot (WB). GTX636650

GTX636650 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Lateral Flow (Lateral Flow). GTX636650

GTX636650 Lateral Flow Image

Detection of respiratory syncytial virus type A nucleoprotein (GTX136751-pro) (0.5 μg/ml) by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636650

GTX636650 ICC/IF Image

Respiratory Syncytial virus Nucleoprotein antibody [HL1248] detects Respiratory Syncytial virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Respiratory Syncytial virus Nucleoprotein stained by Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1248] used in Lateral Flow (Lateral Flow). GTX636650

GTX636650 Lateral Flow Image

Detection of Respiratory Syncytial virus Nucleoprotein by lateral flow assay using the monoclonal antibody pair.

Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) [HL1248])
Detection: Respiratory Syncytial virus Nucleoprotein antibody (GTX636648) [HL1246])

Samples (25 ng) :
1. RSV type A Nucleoprotein (GTX136751-pro)
2. RSV type B Nucleoprotein (GTX136931-pro)
3. SARS-CoV-2 Nucleocapsid protein (GTX135592-pro)
4. FluA (H1N1) Nucleoprotein (GTX135904-pro)
5. FluA (H3N2) Nucleoprotein (GTX135903-pro)
6. FluB Nucleoprotein (GTX136277-pro)
7. Lysis buffer

REFERENCE

There are currently no references for Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650). Be the first to share your publications with this product.

REVIEW

Respiratory Syncytial virus Nucleoprotein antibody [HL1248] Cat. No. GTX636650

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-Jul-2022
Score :
Application Tested : IP
Sample Species : RSV
Sample : HepG2 cells with/without RSV (A Long Strain) infection, MOI 1, 24hrs
Figure Note :
The image is from a collaborating laboratory.
Date : Anonymous submitted on 10-Jun-2022
Score :
Application Tested : ICC/IF
Sample Species : RSV
Sample : HepG2 cells with/without mCherry-RSV (A Long Strain) infection, MOI 1, 24hrs
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 3% BSA and 0.1% Triton in PBS
Lane Description :
Green : Primary antibody
Dilution : 1:1000
Figure Note :
The image is from a collaborating laboratory.
Date : Anonymous submitted on 10-Jun-2022
Score :
Application Tested : WB
Sample Species : RSV
Sample : HepG2 cells with/without RSV (A Long Strain) infection, MOI 1, 24hrs
Amount used : 20μg
Blocking : 5% milk in PBS-Tween 0.02%
Lane Description : Dilution : 1:1000
Figure Note :
The image is from a collaborating laboratory.
Package List Price ($)
$ 399
$ 169
Custom Conjugations