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Respiratory Syncytial virus Nucleoprotein antibody [HL1296]

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in Western Blot (WB). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in Western Blot (WB). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in Western Blot (WB). GTX636710

Cat. No. GTX636710

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1296

Isotype

IgG

Application

WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

Respiratory syncytial virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Respiratory syncytial virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Respiratory Syncytial virus Nucleoprotein. (Strain A2)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910052

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710

GTX636710 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710

GTX636710 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant HEK293 expressed, N-terminal respiratory syncytial virus type A nucleoprotein (50 ng). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (160000-1.9*10-2 pM) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
3. Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710

GTX636710 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710

GTX636710 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture:Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP
Pair 2:
Capture:Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP
Pair 3:
Capture:Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.d with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710

GTX636710 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710

GTX636710 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in Western Blot (WB). GTX636710

GTX636710 WB Image

Respiratory syncytial virus lysate (1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in Western Blot (WB). GTX636710

GTX636710 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in Western Blot (WB). GTX636710

GTX636710 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

Respiratory Syncytial virus Nucleoprotein antibody [HL1296] Cat. No. GTX636710

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Jun-2022
Score :
Application Tested : ICC/IF
Sample Species : RSV
Sample : HepG2 cells with/without mCherry-RSV (A Long Strain) infection, MOI 1, 24hrs
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 3% BSA and 0.1% Triton in PBS
Lane Description :
Green : Primary antibody
Dilution : 1:1000
Figure Note :
The image is from a collaborating laboratory.
Date : Anonymous submitted on 10-Jun-2022
Score :
Application Tested : WB
Sample Species : RSV
Sample : HepG2 cells with/without RSV (A Long Strain) infection, MOI 1, 24hrs
Amount used : 20μg
Blocking : 5% milk in PBS-Tween 0.02%
Lane Description : Dilution : 1:1000
Figure Note :
The image is from a collaborating laboratory.
Package List Price ($)
$ 399
$ 169
Custom Conjugations