Untreated (–) and treated (+) K562 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Ret (phospho Tyr1062) antibody (GTX133941) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.