APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89607-PEP).
Product Note
This antibody is expected to recognize all three reported isoforms (NP_000955.1; NP_001019980.1; NP_001138774.1 ). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar.
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SPSLSPSSHRSSPATQSP, from the C Terminus of the protein sequence according to NP_000955.1; NP_001138773.1; NP_001019980.1; NP_001138774.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
retinoic acid receptor alpha , NR1B1 , RAR
Cellular Localization
Nucleus , Cytoplasm
Background
This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq, Sep 2010]
Database
Research Area
DATA IMAGES
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GTX89607 IHC-P Image
IHC-P analysis of human cerebellum using GTX89607 Retinoic Acid Receptor alpha antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml
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GTX89607 WB Image
WB analysis of human cerebellum lysate using GTX89607 Retinoic Acid Receptor alpha antibody, C-term.
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for Retinoic Acid Receptor alpha antibody, C-term (GTX89607). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Retinoic Acid Receptor alpha antibody, C-term (GTX89607). Be the first to share your experience with this product.
