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Retinoic Acid Receptor alpha antibody - ChIP grade

Anti-Retinoic Acid Receptor alpha antibody - ChIP grade used in ELISA (ELISA). GTX60338
Anti-Retinoic Acid Receptor alpha antibody - ChIP grade used in Western Blot (WB). GTX60338
Anti-Retinoic Acid Receptor alpha antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60338

Cat No. GTX60338

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA, ChIP assay

Reactivity

Human, Mouse
Package
50 μl ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:750
ELISA 1:50
ChIP assay 1-10 μl
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Human RARA (Retinoic Acid Receptor alpha) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

retinoic acid receptor alpha , NR1B1 , RAR

Cellular Localization

Nucleus , Cytoplasm

Background

This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES

Anti-Retinoic Acid Receptor alpha antibody - ChIP grade used in ELISA (ELISA). GTX60338

GTX60338 ELISA Image

ELISA analysis of peptides used for immunization using GTX60338 Retinoic Acid Receptor alpha antibody - ChIP grade.

Anti-Retinoic Acid Receptor alpha antibody - ChIP grade used in Western Blot (WB). GTX60338

GTX60338 WB Image

WB analysis of 293T transfected with a RARA construct (lane 2) or with a negative control construct (lane 1) using GTX60338 Retinoic Acid Receptor alpha antibody - ChIP grade.
Dilution : 1:750

Anti-Retinoic Acid Receptor alpha antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60338

GTX60338 ChIP assay Image

ChIP analysis of sheared chromatin from 6x10⁶ NB4 cells using GTX60338 Retinoic Acid Receptor alpha antibody - ChIP grade. QPCR was performed using primers specific for the TGM2, HMHA1, PRAM1 and H2B genes. This figure shows the relative occupancy, calculated as the ratio + control/background for which the second exon of the MB gene was used.
Antibody amount : 4μl

REFERENCE

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REVIEW

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Package List Price ($)
$ 289