APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:750 |
| 1:50 |
| 1-10 μl |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Serum
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Human RARA (Retinoic Acid Receptor alpha) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
retinoic acid receptor alpha , NR1B1 , RAR
Cellular Localization
Nucleus , Cytoplasm
Background
This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq, Sep 2010]
Database
Research Area
DATA IMAGES
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GTX60338 ELISA Image
ELISA analysis of peptides used for immunization using GTX60338 Retinoic Acid Receptor alpha antibody - ChIP grade.
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GTX60338 ChIP assay Image
ChIP analysis of sheared chromatin from 6x10⁶ NB4 cells using GTX60338 Retinoic Acid Receptor alpha antibody - ChIP grade. QPCR was performed using primers specific for the TGM2, HMHA1, PRAM1 and H2B genes. This figure shows the relative occupancy, calculated as the ratio + control/background for which the second exon of the MB gene was used.
Antibody amount : 4μl
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GTX60338 WB Image
WB analysis of 293T transfected with a RARA construct (lane 2) or with a negative control construct (lane 1) using GTX60338 Retinoic Acid Receptor alpha antibody - ChIP grade.
Dilution : 1:750
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REFERENCE
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REVIEW
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