APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:250 |
| Assay dependent |
| 1-2 μl |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody detects RAR gamma-1, but not RAR gamma-2, or either of the alpha or beta forms of RAR.
PROPERTIES
Form
Liquid
Buffer
Serum diluted with PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Bovine
Immunogen
Synthetic peptide corresponding to the residues E(40) M L S P S F R G L G Q P D L P K E(57) of the N-terminal end of RAR gamma-1.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
retinoic acid receptor gamma , NR1B3 , RARC
Cellular Localization
Nucleus
Background
This gene encodes a retinoic acid receptor that belongs to the nuclear hormone receptor family. Retinoic acid receptors (RARs) act as ligand-dependent transcriptional regulators. When bound to ligands, RARs activate transcription by binding as heterodimers to the retinoic acid response elements (RARE) found in the promoter regions of the target genes. In their unbound form, RARs repress transcription of their target genes. RARs are involved in various biological processes, including limb bud development, skeletal growth, and matrix homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Database
Research Area
DATA IMAGES
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GTX25904 WB Image
WB analysis of human HEK293 cells using GTX25904 Retinoic Acid Receptor gamma antibody.
Lane 1 : Primary antibody with blocking peptide
Lane 2 : Primary antibody
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GTX25904 WB Image
WB analysis of tissue extract (30 μg lysate) of rat brain using GTX25904 Retinoic Acid Receptor gamma antibody.
Dilution : 1:250
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GTX25904 WB Image
WB analysis of various cell lysates using GTX25904 Retinoic Acid Receptor gamma antibody.
Loading : 50 μg
Dilution : 1:500
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REFERENCE
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REVIEW
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