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Retinoid X Receptor alpha antibody

Anti-Retinoid X Receptor alpha antibody used in Western Blot (WB). GTX113828
Anti-Retinoid X Receptor alpha antibody used in Immunoprecipitation (IP). GTX113828
Anti-Retinoid X Receptor alpha antibody used in Western Blot (WB). GTX113828

Cat. No. GTX113828

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Retinoid X Receptor alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HepG2 , rat liver

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Retinoid X Receptor alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11179413

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

retinoid X receptor alpha , NR2B1

Cellular Localization

Nucleus

Background

Retinoid X receptors (RXRs) and retinoic acid receptors (RARs), are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors exert their action by binding, as homodimers or heterodimers, to specific sequences in the promoters of target genes and regulating their transcription. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Retinoid X Receptor alpha antibody used in Western Blot (WB). GTX113828

GTX113828 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX113828 diluted at 1:5000

Anti-Retinoid X Receptor alpha antibody used in Immunoprecipitation (IP). GTX113828

GTX113828 IP Image

Retinoid X Receptor alpha antibody immunoprecipitates RXRA protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of RXRA protein by 4 μg Retinoid X Receptor alpha antibody (GTX113828)
7.5 % SDS-PAGE
The immunoprecipitated RXRA protein was detected by Retinoid X Receptor alpha antibody (GTX113828) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-Retinoid X Receptor alpha antibody used in Western Blot (WB). GTX113828

GTX113828 WB Image

Rat tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Retinoid X Receptor alpha antibody (GTX113828) diluted at 1:500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169