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Retinoid X Receptor alpha antibody

Anti-Retinoid X Receptor alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113829
Anti-Retinoid X Receptor alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113829

Cat. No. GTX113829

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Retinoid X Receptor alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Retinoid X Receptor alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885334

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

retinoid X receptor alpha , NR2B1

Cellular Localization

Nucleus

Background

Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-Retinoid X Receptor alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113829

GTX113829 ICC/IF Image

Retinoid X Receptor alpha antibody detects Retinoid X Receptor alpha protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Retinoid X Receptor alpha protein stained by Retinoid X Receptor alpha antibody (GTX113829) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-Retinoid X Receptor alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113829

GTX113829 IHC-P Image

Retinoid X Receptor alpha antibody detects Retinoid X Receptor alpha protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Retinoid X Receptor alpha antibody (GTX113829) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Yang YSH et al. Sci Rep 2020; 10 (1) : 866 Long-term Proton Pump Inhibitor Administration Caused Physiological and Microbiota Changes in Rats.
Application : IHC-P
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169