GeneTex
United States (US)

Rex1 antibody [N1C1]

GTX101903_39827_CT_IFA_mESC2_19011506_502.jpg
GTX101903_39827_IFA_mESC_19011506_920.jpg
GTX101903_39827_WB_hESC_19011506_204.jpg
GTX101903_43244_20180704_ICC_IF_19011506_326.jpg

Cat No. GTX101903

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Human ESC
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rex1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

REX1 antibody, ZNF754 antibody, ZFP42 antibody, zinc finger protein 42 homolog antibody, zinc finger protein 754 antibody, REX1 transcription factor antibody, reduced expression protein 1 antibody, hREX-1 antibody, zfp-42 antibody, REX-1 antibody, zinc finger protein 42 homolog (mouse) antibody

Cellular Localization

Nucleus

Background

Involved in self-renewal property of ES cells (By similarity). May be involved in transcriptional regulation.

Database

Research Area

DATA IMAGES
GTX101903_39827_CT_IFA_mESC2_19011506_502.jpg

GTX101903 ICC/IF Image

Immunofluorescence analysis of mESC-KH2 p17 on MEF feeder, using Rex1(GTX101903) antibody at 1:332 dilution.

GTX101903_39827_IFA_mESC_19011506_920.jpg

GTX101903 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Rex1(GTX101903) antibody at 1:100 dilution.

GTX101903_39827_WB_hESC_19011506_204.jpg

GTX101903 WB Image

Sample (20 µg of whole cell lysate)
A: Human ESC
10% SDS PAGE
GTX101903 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101903_43244_20180704_ICC_IF_19011506_326.jpg

GTX101903 ICC/IF Image

Rex1 antibody [N1C1] detects Rex1 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rex1 stained by Rex1 antibody [N1C1] (GTX101903) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10μm.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169