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Rfa2 (Pan Reactive) antibody

Anti-Rfa2 (Pan Reactive) antibody used in Western Blot (WB). GTX48746

Cat. No. GTX48746

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA

Reactivity

Yeast
Package
50 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100 - 1:500
IP 1:100
ELISA 1:1000 - 1:3000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Yeast

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2).?? The RFA complex consists of a heterotrimer of

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rfa2p , BUF1 , RPA2 , RPA32

Cellular Localization

Nucleus

Database

DATA IMAGES

Anti-Rfa2 (Pan Reactive) antibody used in Western Blot (WB). GTX48746

GTX48746 WB Image

This product was assayed by western blotting against RFA2 containing cell lysates. Blot incubated for 1 hr at RT with affinity purified Rabbit anti-RFA2 pan reactive Lot 13229 at 1.0 μg/ml followed by 1:5000 dilution of IRDye800 sheep anti-Rabbit IgG for 1 hr at RT.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399