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Rfa2 (Pan Reactive) antibody

Anti-Rfa2 (Pan Reactive) antibody used in Western Blot (WB). GTX48746

Cat No. GTX48746

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA

Reactivity

Yeast
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100 - 1:500
IP 1:100
ELISA 1:1000 - 1:3000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Yeast

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2).?? The RFA complex consists of a heterotrimer of

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rfa2p , BUF1 , RPA2 , RPA32

Cellular Localization

Nucleus

Database

DATA IMAGES

Anti-Rfa2 (Pan Reactive) antibody used in Western Blot (WB). GTX48746

GTX48746 WB Image

This product was assayed by western blotting against RFA2 containing cell lysates. Blot incubated for 1 hr at RT with affinity purified Rabbit anti-RFA2 pan reactive Lot 13229 at 1.0 μg/ml followed by 1:5000 dilution of IRDye800 sheep anti-Rabbit IgG for 1 hr at RT.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 289